This research work represents an experimental study of the interaction between water drops deposited on a substrate at ambient temperature. To examine this phenomenon, the evaporation of a single drop deposited on a substrate was first investigated. Then, several drops were deposited alongside on the same substrate under the same conditions. The central drop dynamic behavior was also examined and compared with that of a single drop. This comparison shows the effect of the interaction between the neighboring drops, which delayed the evaporation of these drops and particularly the central droplet, on evaporation. In fact, three configurations were studied by changing the initial distance (d) between the drops (d = 0.2 mm, d = 7 mm and d = 15 mm). The obtained results reveal that the interaction phenomenon becomes less important by increasing the distance between the drops. This is important for optimizing many industrial applications, such as spray drying, fuel injection in combustion engines, and other applications.