In this paper, an observer with exponential time-varying gain is proposed for a class of nonlinear systems in Takagi–Sugeno form with both continuous-time and aperiodic sampled outputs. The proposed observer consists of two parts: one related to the continuous-time outputs and one related to the discrete outputs. Sufficient conditions involving a tuning parameter and the maximum allowed sampling time are derived in terms of quasi linear matrix inequality with respect to a scalar to ensure asymptotic convergence of the observer on the basis of Lyapunov–Krasovskii functional and approach. Numerical simulations are provided to show the benefits of the proposed observer. Some comparisons with the high gain continuous-discrete observer and fuzzy observer with constant gain are provided to illustrate the interest of the proposed scheme.